An agar medium; DNase agar, a differential medium is used to test the ability of an organism to produce deoxyribonuclease or DNase. If the reagent turns red, the indole test is positive. Starch hydrolysis test: Serratia marcescens-Lactase test: Staphylococcus aureus + Lactase test: Streptococcus faecalis + Lactase test: Micrococcus luteus-Lactase test: Bacillus subtilis + Lactase test: Escherichia coli + Lactase test: Enterobacter aerogenes + Lactase test: Pseudomonas fluorescens- The Kovac’s reagent that you add to the SIM medium to test for indole contains hydrochloric acid, p-dimethylaminobenzaldehyde (DMABA), and n-amyl alcohol. SALISBURYANDJOSEPHJ. 4. Serratia marcescens: Serratia odorifera biogroup1: Serratia odorifera biogroup2 ... Providencia: Morganella: Serratia marcescens. Bizio named the genus Serratia in honor of and Italian physicist named Serratia, and chose marcescens for the species name after the … Salmonella enterica KCN growth neg. (It is this one) Edwardsiella tarda Proteus vulgaris Morganella morganii Providencia stuartii Indole Test Urease H2S?? Provide a flow chart/dichotomous key to all tests you did for your organism. About Organism: Show All Tests : Show Unique Test Hierarchy: Description of values of test status. J. clin. Salmonella. It was discovered in 1819 by Bartolomeo Bizio in Padua, Italy. Serratia marcescens Serratia liquefaciens Shigella sonnei Yersinia pestis Serratia marcescens Red pigment Serratia liquefaciens No Red pigment Salmonella bongori KCN growth pos. Post author By a b; ... test, decarboxylation, deamination,Bile Esculin, Starch hydrolysis, Urea, Casein, Gelating hydrolysis, DNA hydrolysis, Lipid hydrolysis, SIM and any other test you consider appropriate in the identification of your organism. Serratia marcescens is a motile,short rod-shaped, Gram-negative, facultative anaerobe bacterium, classified as an opportunistic pathogen. DNA hydrolysis test or Deoxyribonuclease (DNase) test is used to determine the ability of an organism to hydrolyze DNA and utilize it as a source of carbon and energy for growth. Before the Kovac's reagent has been added: Path., 1972, 25, 1083-1085 Hydrolysis ofcasein: a differential aid for the indentification ofSerratia marcescens WILLIAMA. Description and Significance. DMABA reacts with indole to produce a red quinoidal compound. DNase agar is a differential medium that tests the ability of an organism to produce an exoenzyme, called deoxyribonuclease or DNase, that hydrolyzes DNA. Serratia marcescens Morganella morganii Bacillus subtilis. Gram negative rod starch negatve ... Starch Hydrolysis test. Methyl green is a cation which binds to the negatively-charged DNA. Clearing of agar around bacterial growth is an evidence that bacteria secretes casein, protein capable of digesting casein Un-inoculated milk agar Bacillus subtilis Seccatia marcescens Starch Hydrolysis test Starch hydrolysis test assesses ability of bacteria to secrete an enzyme amylase. LIKOS Fromthe Veterans Administration Hospital, Phoenix, Arizona, USA SYNOPSIS Through the hydrolysis ofcasein, a cultural characteristic has been established in the preliminary identification of Serratia marcescens. DNase Test. Amylase hydrolyses starch to glucose in surrounding media. Serratia marcescens. Serratia Marcescens. The oxidase test is based on the bacterial production of an intracellular oxidase enzyme. Gram negative rod starch negative lactose negative Mr negative VP positive citrate positive indole negative motility positive urease negative gelatin hydrolysis positive H2S negative nitrate positive oxidase negative. • *starch hydrolysis test (amylase) • *PR-glucose, PR-lactose, PR-sucrose tests (fermentation) ... Serratia marcescens Shigella flexneri Gram-positive Bacillus cereus Bacillus megaterium Bacillus subtilis Enterococcus faecalis Lactococcus lactis Micrococcus luteus DNase agar contains nutrients for the bacteria, DNA, and methyl green as an indicator.